THE ULTIMATE GUIDE TO GENETIC METHYLATION TEST GARY BREAKA

therapy with sodium bisulfite deaminates unmethylated cytosine to uracil, While 5mC will not be influenced. Subsequent PCR amplification will exchange the uracils by thymines. Reads from NGS libraries made in parallel with and without bisulfite procedure are mapped onto reference genomes plus the contrasting C/T positions among the libraries point